The Study On Morphological And Morphometric Analysis Of Sacral Hiatus In Dry Human Sacra

This study was carried out on 80 dry human sacra in meenakshi medical college Anatomical variations inmorphology and morphometry of sacral hiatus. Various shapes of sacral hiatus were observed which includedinverted U (66.3%), inverted V (11.3%), irregular (11.3%), dumbbell (3.8%) Agenesis Of the dorsal (6.3%) and bifid(1.3%). The apex of sacral hiatus was commonly found at the level of 4th sacral vertebra in 76.0%. the meanlength of sacral hiatus was 22.9 mm. The mean anteroposterior diameter of sacral canal at the apex of sacralhiatus was 4.97 mm. Narrowing of sacral canal at the apex of sacral hiatus, diameter less than 4mm, wasobserved to be significantly high, (36%). The knowledge of anatomical variations of sacral hiatus is significantwhile administration of caudal epidural anaesthesia and it may help to improve its success rate

Use of eschar for the molecular diagnosis and genotypic characterisation of Orientia tsutsugamushi causing scrub typhus

Scrub typhus caused by Orientia tsutsugamushi presents as an acute febrile illness with a varied presentation from mild illness to fatal disease in the absence of appropriate antibiotic treatment. Performing polymerase chain reaction (PCR) on eschar sample acts a rapid diagnostic tool in the early stage of scrub typhus when blood is negative. A total of eight patients from whom both whole blood and eschar samples were collected and tested by nested PCR targeting 56 kDa trichostatin A (TSA) gene to detect O. tsutsugamushi DNA. All (100%) eschar samples and three whole blood samples tested positive. Genetic analysis of the 56 kDa TSA gene sequences showed that the majority were related to Karp reference strains, while one clustered with Kawasaki strain. When present, eschar should be favoured as a diagnostic sample over whole blood in the early phase of infection.

A Rare Clinical Phenomenon of Six Distomolars: A Case Report.

Th e report presents a case of a rarely occurring totally impacted two bilateral maxillary distomolar and single bilateral mandibular distomolar. Th e appearance of supernumerary teeth is a relatively uncommon dental anomaly and it is rare for patients to have six impacted distomolars. Th e aim of this case report is to describe the presence of bilateral distomolars in the maxilla and mandible in a young female patient aged 19 years. Orthopantomogram revealed impacted six bilateral distomolars. Following orthodontic treatment, the patient was referred for removal of distomolars along with all the four impacted third molars under local anesthesia.

Extra Hilar Branching of Renal Arteries: An Anatomical Study.

Introduction: The objective of this study was to observe the patterns of different arteries that supply the kidneys. The kidney has a segmental distribution of arteries. The kidneys are divided into five vascular segments. The arteries that arise from the aorta above or below the main renal artery and reach the hilum are called accessory renal arteries. They are persistent embryonic lateral splanchnic arteries. Accessory renal arteries may arise from the celiac or superior mesenteric arteries, near the bifurcation or from the common iliac arteries. The present study has attempted to find out accessory, and aberrant arteries to kidneys with review of literature. Materials and Methods: The study was done on 52 kidneys randomly selected from cadavers that were used for the purpose of teaching in the department of Anatomy at P.E.S Medical College. The kidneys were removed from the cadavers en-block with the arteries and veins intact. The renal artery was observed for its pattern of branching. Observations and Discussion: The pre-hilar branching pattern was absent only in six kidneys out of the 52 kidneys selected. The branches given before entering the hilum were either in the form of a fork pattern or a ladder pattern in the remaining 46 kidneys. The fork pattern wherein the branches arose from a single point was found in 42 kidneys. The ladder patterns were seen in two posterior segment arteries and two anterior segment arteries. The anterior division often showed the fork patterns which were either duplicate or triplicate outside the hilum more proximally, with further division into duplicate or triplicate terminal branches closer to the hilum but significantly outside.

Eco-Friendly Approach for Treating Dairy Effluent and Lipid Estimation Using Microalgae.

Aim: In this study, an attempt has been made to examine the utility of these species in treating dairy wastewater. Bioreactor was studied using immobilized Chlorella vulgaris and Anabaena ambigua to treat dairy effluent. Study Design: The entire study including the treatment and filtration was conducted in Centre for biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, between November 2014 to February 2015. Methodology: The treatment of dairy effluent consists of two stages; the first stage includes dairy effluent treatment using immobilised Chlorella vulgaris and Anabeana ambigua, while the second stage involves sand bed and coal bed filtration. Results: Chlorella vulgaris reported a high lipid content of 12% when compared with Anabaena ambigua 5% after the cultivation period. Whereas the protein content of Anabaena ambigua (40%) was higher than Chlorella vulgaris (28%) when compared with after harvesting. Whilst ammonium nitrate was completely removed by bead treatment it was 96% reduction when treated with Chlorella vulgaris. A 98% removal of phosphates was achieved on an average after algal bead treatment for both species in both modes of operation. Moreover, a significant decrease in BOD and COD was achieved by this method. Conclusion: It can be concluded that, the cultivation of algae in dairy effluent results the combined advantages of treating the dairy effluent and also producing algal biomass, which can further use of food additives for aquatic culture, animal and human feed, energies such as biogas and fuels and bio-fertiliser.

Unusual branching pattern of external carotid artery in the left carotid triangle.

During the routine dissection of the neck for the first MBBS students, we have found the abnormal branching of the external carotid artery in the carotid triangle. The external carotid artery is a terminal branch of the common carotid artery arises at the level of superior border of the thyroid cartilage in the carotid triangle. It supplies the structures of head and neck regions by its eight branches. The knowledge of variations is very important for the general, head & neck, ENT and oncosurgeons, to avoid unforeseen complication in the form of bleeding by injuring the abnormal arteries. It is also important for the vascular surgeons and radiologists while performing the procedures on the arteries.

Brachiocephalic Trunk and Left Common Carotid Artery Arising from Aortic Arch as a Single Trunk.

Branches of aortic arch usually are brachiocephalic trunk, left common carotid artery and left subclavian artery from right to left direction. Arch of aorta show more variations in branching patterns. One among which is origin of only two branches in which brachiocephalic trunk and left common carotid artery arise together as a common trunk and other branch is left subclavian artery. The variations occur due to abnormal development of arch of aorta. During routine dissection of superior mediastinum of thoracic region, we have found the left common carotid artery and brachiocephalic trunk was arising from the arch of aorta as a common trunk. These kinds of variations are very rare and knowledge of which is very important for neck surgeons, cardiologists and interventional radiologists while doing surgeries and instrumentation procedures.

Trifurcation of External Carotid Artery and Variant Branches of First Part of Maxillary Artery.

The external carotid artery normally divides into two terminal branches at the level of the neck of the mandible. The terminal branches are usually the superficial temporal and maxillary arteries. The maxillary artery is described to be in three parts in relation to the lateral pterygoid muscle as the mandibular (first), pterygoid (second) and the pterygopalatine (third) parts. The second part passes behind the muscle. The branches that arise from the first part of the maxillary artery are the deep auricular, anterior tympanic, the middle meningeal, accessory meningeal and inferior alveolar arteries. The middle meningeal artery normally arises at the lower border of lateral pterygoid muscle from the first part of maxillary artery. It then ascends upwards, passes between the two roots of the auriculotemporal nerve and enters the foramen spinosum in the base of skull. During routine dissection of a male cadaver in the department of anatomy while teaching medical students variations were observed in the termination of the external carotid artery on the right side. The artery gave three branches at the termination, superficial temporal, maxillary and between the two the middle meningeal artery was seen arising close to the end of the external carotid artery. The middle meningeal artery did not pass between the two roots of the auriculotemporal nerve. The branches of first part of maxillary artery were variable. The deep auricular branch was absent and its territory may have been supplied by the posterior auricular and anterior auricular arteries. The anterior tympanic and accessory meningeal arteries arose from the middle meningeal artery. There were two inferior alveolar arteries 1.5 cm apart arising from the first part of maxillary artery. The first artery went to the mandibular canal along with the inferior alveolar nerve. The second artery accompanied the lingual nerve to the last molar tooth. Probably this artery may have been an additional supply to the gingiva around the last molar tooth. The other variations that were noted were the absence of mylohyoid branch from the inferior alveolar artery. To the best of our knowledge these variations in the arteries have not been previously reported.

Cytomorphology of subcutaneous panniculitic T-cell lymphoma (SPTCL)--a case report.

T cell lymphomas account for approximately 60% of cutaneous lymphomas. The annual incidence of cutaneous lymphoma is estimated to be from 0.5 to 1 per 1,00,000 persons per year. We present one case of cutaneous lymphoma, an eighteen year old male who presented with multiple swellings all over the body of one month duration. On examination, multiple, subcutaneous, mobile, non tender nodules were seen ranging from 0.5 cm to 5 cm in diameter. FNAC revealed non-Hodgkin's lymphoma morphologically in favour of cutaneous T-cell lymphoma. A biopsy and immunohistochemistry confirmed SPTCL.

Bronchioloalveolar carcinoma mimicking miliary tuberculosis.

A case of bronchioloalveolar carcinoma, with widespread dissemination to both lungs and miliary mottling on chest X-ray is reported in a 40 year old male.

Enzyme inhibitors from plants. Isolation and characterization of a protease inhibitor from arrow root (Maranta arundinaceae) tuber.

A protease inhibitor from arrow root (Maranta arundinaceae) tuber has been isolated in a homogeneous form. The inhibitor has a Mr of 11,000-12,000; it inhibited bovine trypsin, bovine enterokinase, bovine α-chymotrypsin and the proteolytic activity of human and bovine pancreatic preparations. The inhibitor is resistant to pepsin, and elastase. It could withstand heat treatment at 100°C for 60 min and exposure to a wide range of pH (1.0-12.5) for 72 h at 4°C without loss of activity. Arginyl groups are essential for the action of the inhibitor. Preincubation of the inhibitor at pH 3.7 with trypsin or chymotrypsin caused nearly a two-fold increase in inhibitor potency. Keywords. Arrow root tuber; protease